| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PAOX;PAO;Peroxisomal N;1;-acetyl-spermine/spermidine oxidase;Polyamine oxidase |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from PAOX . at AA range: 260-340 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PAOX Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PAOX. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | PAOX |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | PAOX Polyclonal Antibody detects endogenous levels of PAOX protein. |
| Molecular Weight | Observed: 70 kDa |
| UniProt / Swiss-Prot | Q6QHF9 (Human) | Q8C0L6 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:40000;IF 1:50-200
Immunogen / Antigen
Synthesized peptide derived from PAOX . at AA range: 260-340
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
catalytic activity:N(1),N(12)-diacetylspermine + O(2) + H(2)O = N(1)-acetylspermidine + 3-acetamidobutanal + H(2)O(2).,catalytic activity:N(1)-acetylspermidine + O(2) + H(2)O = putrescine + 3-acetamidopropanal + H(2)O(2).,catalytic activity:N(1)-acetylspermine + O(2) + H(2)O = spermidine + 3-acetamidopropanal + H(2)O(2).,cofactor:Binds 1 FAD per subunit.,function:Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.,induction:By polyamine analogs.,miscellaneous:Oxidizes N(1)-acetylated polyamines on the exo-side of their N(4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N(4)-nitrogen.,pathway:Amine and polyamine metabolism; spermine metabolism.,similarity:Belongs to the flavin monoamine oxidase family.,subunit:Monomer.,tissue specificity:Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:40000;IF 1:50-200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
