U-Blot® Paxillin (Phospho Tyr31) Rabbit Polyclonal Antibody

SKU:BHA21820531
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Paxillin antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP9253-50 50 uL
WP9253-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Paxillin
Antigen
  • Paxillin
Alternative names PXN;Paxillin
Gene ID 5829 (Human) | 19303 (Mouse) | 360820 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr31. AA range:15-64
Conjugate
  • Unconjugated
Molecular weight Observed: 68 kDa
Purification Affinity Purified
Cellular localization Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP9253
Main SKU BHA21820531
Antibody

Product Overview

U-Blot® Paxillin (Phospho Tyr31) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Paxillin. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target Paxillin
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Phospho-Paxillin (Y31) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at Y31.
Molecular Weight Observed: 68 kDa
UniProt / Swiss-Prot P49023 (Human) | Q8VI36 (Mouse) | Q66H76 (Rat)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr31. AA range:15-64

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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