| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PCNA;Proliferating cell nuclear antigen;PCNA;Cyclin |
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| Isotype | |
| Reactivity | |
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| Conjugate | |
| Molecular weight | |
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Product Overview
U-Blot® PCNA Mouse Monoclonal Antibody (12D10), AbFluor 350 Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PCNA. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry and reacts with Human, Rat, Mouse.
Validated Applications
| Target | PCNA |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12D10 |
| Isotype | IgG1 |
| Conjugate | AbFluor 350 |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | PCNA Monoclonal Antibody(12D10) AbFluor™ 350 Conjugated specially designed for your Immunofluorescence analysis. |
| Molecular Weight | Observed: 30-33 kDa |
| UniProt / Swiss-Prot | P12004 (Human) |
Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.
Immunogen / Antigen
Proliferating cell nuclear antigen
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: AbFluor 350.
Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Clone 12D10.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
