| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating Cell Nuclear Antigen. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human PCNA protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PCNA Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PCNA. It has been validated for Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and reacts with Human, Mouse, Monkey, Rat, Hamster.
Validated Applications
| Target | PCNA |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Monkey, Rat, Hamster |
| Applications | Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of PCNA and does not cross-react with related proteins. |
| Molecular Weight | Observed: 30-33 kDa |
| UniProt / Swiss-Prot | P12004 (Human) | P17918 (Mouse) |
Recommended dilutions: WB 1:1000;ICC 1:100
Immunogen / Antigen
Purified recombinant human PCNA protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Monkey, Rat, Hamster. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry. Recommended dilutions: WB 1:1000;ICC 1:100
Confirmed reactivity: Human, Mouse, Monkey, Rat, Hamster. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
