| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PCNA;Proliferating cell nuclear antigen;PCNA;Cyclin |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PCNA Rabbit Polyclonal Antibody (WP13893) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PCNA. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Monkey, Rabbit.
Validated Applications
| Target | PCNA |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey, Rabbit |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | PCNA Polyclonal Antibody detects endogenous levels of PCNA protein. |
| Molecular Weight | Observed: 30-33 kDa |
| UniProt / Swiss-Prot | P12004 (Human) | P17918 (Mouse) | P04961 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:100-500;IF/ICC1:100-500;ELISA 1:5000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey, Rabbit. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:100-500;IF/ICC1:100-500;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat, Monkey, Rabbit. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
