| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD antigen CD274 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PD-L1 (ABT015) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD274. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD274 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT015 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 33 kDa | Observed: 50 kDa |
| UniProt / Swiss-Prot | Q9NZQ7 (Human) | Q9EP73 (Mouse) |
Recommended dilutions: IHC 1:50-1:100;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:50-1:100;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT015.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
