| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | DLAT;DLTA;Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex;mitochondrial;70 kDa mitochondrial autoantigen of primary biliary cirrhosis;PBC;Dihydrolipoamide acetyltransferase component of pyruva |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human PDC-E2 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PDC-E2 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PDC-E2. It has been validated for Western Blot and reacts with Human, Rabbit.
Validated Applications
| Target | PDC-E2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Rabbit |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | PDC-E2 Monoclonal Antibody detects endogenous levels of PDC-E2 protein. |
| Molecular Weight | Calculated: 69 kDa |
| UniProt / Swiss-Prot | P10515 (Human) | Q8BMF4 (Mouse) | P08461 (Rat) |
Recommended dilutions: WB 1:1000-1:2000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human PDC-E2 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Rabbit. Confirm suitability for untested species before use.
Scientific Background
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lact
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000-1:2000;Not yet tested in other applications.
Confirmed reactivity: Human, Rabbit. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
