| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial;Pyruvate dehydrogenase kinase isoform 1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human PDHK1 (Phospho Tyr243) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PDK1 (Phospho Tyr243) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PDK1. Recognizes Phospho. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | PDK1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat PDHK1 (Phospho Tyr243) |
| Molecular Weight | Observed: 50 kDa |
| UniProt / Swiss-Prot | Q15118 (Human) | Q8BFP9 (Mouse) | Q63065 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Immunogen / Antigen
Synthesized peptide derived from human PDHK1 (Phospho Tyr243)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
