U-Blot® PDPN Rabbit Polyclonal Antibody

SKU:BHA21811462
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Podoplanin antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA, Immunohistochemistry with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA, Immunohistochemistry
Options selector
Catalog no. Size
WP16476-50 50 uL
WP16476-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Podoplanin
Antigen
  • PDPN
Gene ID 10630 (Human) | 14726 (Mouse) | 54320 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
  • Immunohistochemistry
Immunogen Synthesized peptide derived from human PDPN
Conjugate
  • Unconjugated
Molecular weight Calculated: 18 kDa
Purification Affinity Purified
Cellular localization [Podoplanin]: Membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane ; Single-pass type I membrane protein . Cell projection, filopodium membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Membrane raft . Apical cell membrane . Basolateral cell membrane . Cell projection, invadopodium . Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles (By similarity). Association to the lipid rafts is required for PDPN-induced epithelial to mesenchymal transition (EMT) (PubMed:21376833). Colocalizes with CD9 in tetraspanin microdomains (PubMed:18541721). Localized at invadopodium adhesion rings in tumor cell. Association to the lipid rafts is essential for PDPN recruitment to invadopodia and ECM degradation (PubMed:25486435). .; [29kDa cytosolic podoplanin intracellular domain]: Cytoplasm, cytosol .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP16476
Main SKU BHA21811462
Antibody

Product Overview

U-Blot® PDPN Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Podoplanin. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.

Validated Applications

Target Podoplanin
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA, Immunohistochemistry
Purification Affinity Purified
Specificity This antibody detects endogenous levels of PDPN at Human/Mouse/Rat
Molecular Weight Calculated: 18 kDa
UniProt / Swiss-Prot Q86YL7 (Human) | Q62011 (Mouse) | Q64294 (Rat)

Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

Immunogen / Antigen

Synthesized peptide derived from human PDPN

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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