| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Perforin-1;P1;Cytolysin;Lymphocyte pore-forming protein;PFP |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Perforin 1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Perforin. It has been validated for Western Blot, Immunohistochemistry, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Perforin |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Perforin 1 Polyclonal Antibody detects endogenous levels of Perforin 1 |
| Molecular Weight | Observed: 61 kDa |
| UniProt / Swiss-Prot | P14222 (Human) | P10820 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-200;ELISA 1:10000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:50-200;ELISA 1:10000-20000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
