| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cytolysin;FLH2;HPLH2;Lymphocyte pore-forming protein;P1;PERF_HUMAN;perforin 1;pore forming protein;Perforin 1;Perforin-1;PFP;PGFL;PIGF;PIGF-2;PLGF;Pore forming protein;prf1;SHGC-10760 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Perforin AA range: 1-100 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Perforin (ABT355) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Perforin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Perforin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT355 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Perforin protein. |
| Molecular Weight | Calculated: 61 kDa | Observed: 70 kDa |
| UniProt / Swiss-Prot | P14222 (Human) | P10820 (Mouse) | P35763 (Rat) |
Recommended dilutions: IHC 1:100-500;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Perforin AA range: 1-100
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Perforin is a specific marker of activated cytotoxic T cells and natural killer cells. It can destroy the corresponding target cell membrane and induce apoptosis. It is mainly expressed in CD3 negative and CD56 positive NK cells, CD3 positive large granular lymphocytes and γδ Type T cells. It is mainly used for the auxiliary diagnosis of NK cell lymphoma and NK cell like T-cell lymphoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-500;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT355.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
