| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MAPK1/MAPK3 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Phospho-ERK 1/2 (Y222/205) at AA range of 140-220 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Phospho ERK 1/2 (Tyr222/205) Mouse Monoclonal Antibody (4G3) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against ERK 1/2. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence and reacts with Human, Rat, Mouse.
Validated Applications
| Target | ERK 1/2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4G3 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | Phospho-ERK 1/2 (Y222/205)protein detects endogenous levels of MAPK1/MAPK3 |
| Molecular Weight | Observed: 44,42 kDa |
| UniProt / Swiss-Prot | P27361;P28482 (Human) |
Recommended dilutions: IHC 1:100-200;IF 1:50-200
Immunogen / Antigen
Synthetic Peptide of Phospho-ERK 1/2 (Y222/205) at AA range of 140-220
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence. Recommended dilutions: IHC 1:100-200;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 4G3.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
