| Field | Specification |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 13, MAP kinase 13, MAPK 13 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
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| Target |
Product Overview
U-Blot® Phospho-p38 MAPK (Thr180/Tyr182) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-p38 MAPK (Thr180/Tyr182) (Phosphorylation [Thr180/Tyr182]). It is designed for detection and analysis of the Phosphorylation [Thr180/Tyr182] post-translational modification across research applications.
p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAPK, p38α, β, γ(also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors. MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. SB203580 is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27. SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:500-1:2000 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 42 kDa | Observed: 43 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse, Rat. It targets the Phosphorylation [Thr180/Tyr182] modification and is suitable for Western Blot.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot. Recommended dilutions: WB 1:500-1:2000.
Use WB 1:500-1:2000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-p38 MAPK (Thr180/Tyr182) (Phosphorylation [Thr180/Tyr182]); reactivity: Human, Mouse, Rat.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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