| Field | Specification |
|---|---|
| Alternative Names | RIP-1, RIP1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-RIPK1(Ser161) Mouse mAb is a recombinant monoclonal antibody raised in Mouse that specifically recognizes Phospho-RIPK1 (Ser161) (Phosphorylation [Ser161]). It is designed for detection and analysis of the Phosphorylation [Ser161] post-translational modification across research applications.
RIPK1 is a Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival. Its Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. In normal conditions, RIPK1 acts as an inhibitor of RIPK3-dependent necroptosis, a process mediated by RIPK3 component of complex IIb, which catalyzes phosphorylation of MLKL upon induction by ZBP1. RIPK1 inhibits RIPK3-mediated necroptosis via FADD-mediated recruitment of CASP8, which cleaves RIPK1 and limits TNF-induced necroptosis.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:500-1:1000 |
| Host | Mouse |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG1/Kappa |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein G |
| Molecular Weight | Calculated: 76 kDa | Observed: 85 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser161] modification and is suitable for Western Blot.
Safety & Handling
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It is validated for Western Blot. Recommended dilutions: WB 1:500-1:1000.
Use WB 1:500-1:1000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-RIPK1 (Ser161) (Phosphorylation [Ser161]); reactivity: Human.
The recombinant monoclonal antibody (Mouse host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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