| Field | Specification |
|---|---|
| Alternative Names | RPS6, RS6, S6 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho S6 Ribosomal Protein (Ser235/236) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-S6 Ribosomal Protein (Ser235/Ser236) (Phosphorylation [Ser235/Ser236]). It is designed for detection and analysis of the Phosphorylation [Ser235/Ser236] post-translational modification across research applications.
Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy terminal region of S6 protein.
Validated Applications
| Application | Western Blot, Immunofluorescence |
|---|---|
| Recommended Dilution | WB 1:500-1000, ICC/IF 1:100-300 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 32 kDa | Observed: 32 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse. It targets the Phosphorylation [Ser235/Ser236] modification and is suitable for Western Blot, Immunofluorescence.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:500-1000, ICC/IF 1:100-300.
Use WB 1:500-1000, ICC/IF 1:100-300. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-S6 Ribosomal Protein (Ser235/Ser236) (Phosphorylation [Ser235/Ser236]); reactivity: Human, Mouse.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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