| Field | Specification |
|---|---|
| Alternative Names | YAP65 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-YAP1 (Ser127) Rabbit mAb is a monoclonal antibody raised in Rabbit that specifically recognizes Phospho-YAP1 (Ser127) (Phosphorylation [Ser127]). It is designed for detection and analysis of the Phosphorylation [Ser127] post-translational modification across research applications.
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration.
Validated Applications
| Application | Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry |
|---|---|
| Recommended Dilution | WB 1:500-1:2000, IHC-P 1:100-1:200, ICC/IF 1:50, FC 1:50-1:100 |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 54 kDa | Observed: 70-100 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser127] modification and is suitable for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry.
Safety & Handling
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It is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry. Recommended dilutions: WB 1:500-1:2000, IHC-P 1:100-1:200, ICC/IF 1:50, FC 1:50-1:100.
Use WB 1:500-1:2000, IHC-P 1:100-1:200, ICC/IF 1:50, FC 1:50-1:100. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-YAP1 (Ser127) (Phosphorylation [Ser127]); reactivity: Human.
The monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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