| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PIK3R1;GRB1;Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein of PI3 Kinase P85α |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PI 3 Kinase p85α Mouse Monoclonal Antibody (3B7) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PI3 Kinase P85α. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Rat, Mouse.
Validated Applications
| Target | PI3 Kinase P85α |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3B7 |
| Conjugate | Unconjugated |
| Reactivity | Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | PI3 Kinase P85α protein detects endogenous levels of PI3 Kinase P85α |
| Molecular Weight | Observed: 85 kDa |
| UniProt / Swiss-Prot | P27986 (Human) | P26450 (Mouse) | Q63787 (Rat) |
Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200
Immunogen / Antigen
Recombinant Protein of PI3 Kinase P85α
Reactivity Notes
Confirmed cross-reactivity: Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200
Confirmed reactivity: Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 3B7.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
