| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;5-bisphosphate 3-kinase catalytic subunit alpha isoform;caPI3K;CLOVE;CWS5;MCAP;MCM;MCMTC;MGC142161;MGC142163;p110 alpha;p110alpha;Phosphatidylinositol 3 kinase catalytic alpha polypeptide;Phosphatidylinositol 3 kinase catalytic 110 KD alpha;Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha;Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform;Phosphatidylinositol 4, 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha;Phosphatidylinositol-4;Phosphoinositide 3 kinase catalytic alpha polypeptide;PI 3 Kinase catalytic subunit alpha;PI3 kinase p110 subunit alpha;PI3-kinase subunit alpha;PI3K;PI3K-alpha;PI3KC A;PIK3C A;Pik3ca;PK3CA;PK3CA_HUMAN;PtdIns 3 kinase p110;PtdIns-3-kinase subunit alpha;PtdIns-3-kinase subunit p110-alpha;Serine/threonine protein kinase PIK3CA |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human PIK3CA H1047R AA range: 1000-1068 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PIK3CA H1047R (ABT453) Mouse Monoclonal Antibody (WM1455) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PIK3CA H1047R. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | PIK3CA H1047R |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT453 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize the h1047r mutation of human PIK3CA protein, and does not react with wild-type PIK3CA protein. |
| Molecular Weight | Calculated: 124 kDa | Observed: 124 kDa |
| UniProt / Swiss-Prot | P42336 (Human) | P42337 (Mouse) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human PIK3CA H1047R AA range: 1000-1068
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The PIK3CA gene is located at 3Q26.3 and encodes a 124 kDa protein. The protein is composed of 85 kDa regulatory subunit and 110 kDa catalytic subunit. The most common mutations of PIK3CA occur in exon 9 and exon 20. H1047R, one of the most common mutations occurs in exon 20. PIK3CA plays an important role in the PI3K/AKT/mTOR signaling pathway, which can be activated by this mutation, closely related to the occurrence of tumors and disease progression. In non-small cell lung cancer, H1047R mutantion can phosphorylate Akt and activate downstream pathways to promote the survival and expansion of tumor cells.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT453.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
