| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | LIMS1;PINCH;PINCH1;LIM and senescent cell antigen-like-containing domain protein 1;Particularly interesting new Cys-His protein 1;PINCH-1;Renal carcinoma antigen NY-REN-48 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of human Pinch-1 expressed in E. Coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Pinch-1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Pinch-1. It has been validated for Western Blot, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human.
Validated Applications
| Target | Pinch-1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | Pinch-1 Monoclonal Antibody detects endogenous levels of Pinch-1 protein. |
| Molecular Weight | Calculated: 37 kDa |
| UniProt / Swiss-Prot | P48059 (Human) | Q99JW4 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human Pinch-1 expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
