| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RCHY1;ARNIP;CHIMP;PIRH2;RNF199;ZNF363;RING finger and CHY zinc finger domain-containing protein 1;Androgen receptor N-terminal-interacting protein;CH-rich-interacting match with PLAG1;E3 ubiquitin-protein ligase Pirh2;RING finger |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human Pirh2 expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Pirh2 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Pirh2. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human, Rat.
Validated Applications
| Target | Pirh2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | Pirh2 Monoclonal Antibody detects endogenous levels of Pirh2 protein. |
| Molecular Weight | Calculated: 30 kDa |
| UniProt / Swiss-Prot | Q96PM5 (Human) | Q9CR50 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
