U-Blot® PKD2 (Phospho Ser812) Rabbit Polyclonal Antibody

SKU:BHA21804587
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-PKD2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA, Immunohistochemistry with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA, Immunohistochemistry
Options selector
Catalog no. Size
WP10225-50 50 uL
WP10225-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target PKD2
Antigen
  • Polycystin-2
Alternative names PKD2;Polycystin-2;Autosomal dominant polycystic kidney disease type II protein;Polycystic kidney disease 2 protein;Polycystwin;R48321
Gene ID 5311 (Human) | 18764 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
  • Immunohistochemistry
Immunogen The antiserum was produced against synthesized peptide derived from human PKD2 around the phosphorylation site of Ser812. AA range:778-827
Conjugate
  • Unconjugated
Molecular weight Calculated: 97 kDa
Purification Affinity Purified
Cellular localization Cell projection, cilium membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane . Cytoplasmic vesicle membrane . Golgi apparatus . PKD2 localization to the plasma and ciliary membranes requires PKD1. PKD1:PKD2 interaction is required to reach the Golgi apparatus form endoplasmic reticulum and then traffic to the cilia (By similarity). Retained in the endoplasmic reticulum by interaction with PACS1 and PACS2 (PubMed:15692563). Detected on kidney tubule basolateral membranes and basal cytoplasmic vesicles (PubMed:10770959). Cell surface and cilium localization requires GANAB (PubMed:27259053). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP10225
Main SKU BHA21804587
Antibody

Product Overview

U-Blot® PKD2 (Phospho Ser812) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PKD2. Recognizes Phospho. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.

Validated Applications

Target PKD2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA, Immunohistochemistry
Purification Affinity Purified
Specificity Phospho-PKD2 (S812) Polyclonal Antibody detects endogenous levels of PKD2S812.
Molecular Weight Calculated: 97 kDa
UniProt / Swiss-Prot Q13563 (Human) | O35245 (Mouse)

Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human PKD2 around the phosphorylation site of Ser812. AA range:778-827

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

polycystin 2, transient receptor potential cation channel(PKD2) Homo sapiens This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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