| Field | Specification |
|---|---|
| Target | |
| Alternative names | CTHBP;Cytosolic thyroid hormone binding protein;Cytosolic thyroid hormone-binding protein;KPYM_HUMAN;MGC3932;OIP 3;OIP-3;OIP3;OPA interacting protein 3;Opa-interacting protein 3;p58;PK muscle type;PK, muscle type;PK2;PK3;PKM;PKM2;pykm;Pyruvate kinase 2/3;Pyruvate kinase 3;Pyruvate kinase isozymes M1/M2;Pyruvate kinase muscle;Pyruvate kinase muscle isozyme;pyruvate kinase PKM;Pyruvate kinase, muscle 2;TCB;THBP1;Thyroid hormone binding protein 1;Thyroid hormone binding protein cytosolic;Thyroid hormone-binding protein 1;Tumor M2 PK;Tumor M2-PK. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human PKM2 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PKM2 Mouse Monoclonal Antibody (WM0644) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PKM2. It has been validated for Western Blot, Immunofluorescence and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | PKM2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of PKM2 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | P14618 (Human) | P52480 (Mouse) |
Recommended dilutions: WB 1:1000;ICC 1:400;IF 1:50-200
Immunogen / Antigen
Purified recombinant human PKM2 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000;ICC 1:400;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
