| Field | Specification |
|---|---|
| Alternative Names | PKM;OIP3;PK2;PK3;PKM2;Pyruvate kinase isozymes M1/M2;Cytosolic thyroid hormone-binding protein;CTHBP;Opa-interacting protein 3;OIP-3;Pyruvate kinase 2/3;Pyruvate kinase muscle isozyme;Thyroid hormone-binding protein 1;THBP1;Tu |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKM2. AA range:181-230 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PKM2 Rabbit Polyclonal Antibody (WP14025) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PKM2. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | PKM2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | PKM2 Polyclonal Antibody detects endogenous levels of PKM2 protein. |
| Molecular Weight | Observed: 58 kDa |
| UniProt / Swiss-Prot | P14618 (Human) | P52480 (Mouse) | P11980 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human PKM2. AA range:181-230
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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