| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;PLC-148;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-II;PLC-II;Phospholipase C-gamma-1;PLC-gamma-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phosho peptide around human PLCγ1 (Ser1248) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PLCγ1 (Phospho Ser1248) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PLCG1. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Mouse.
Validated Applications
| Target | PLCG1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human Mouse PLCγ1 (phospho-Ser1248) |
| Molecular Weight | Observed: 150 kDa |
| UniProt / Swiss-Prot | P19174 (Human) | Q62077 (Mouse) | P10686 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
Synthesized phosho peptide around human PLCγ1 (Ser1248)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
