| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PLCG2;1-phosphatidylinositol 4;5-bisphosphate phosphodiesterase gamma-2;Phosphoinositide phospholipase C-gamma-2;Phospholipase C-IV;PLC-IV;Phospholipase C-gamma-2;PLC-gamma-2 |
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Product Overview
U-Blot® PLC γ2 (Phospho Tyr1217) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against PLCG2. Recognizes Phospho (Tyr1217). It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | PLCG2 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1383R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | PLC γ2 (Phospho Tyr1217) Antibody detects endogenous levels of PLC γ2 protein only when phosphorylated at Y1217.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):FLyDT |
| Molecular Weight | Calculated: 148 kDa | Observed: 150 kDa |
| UniProt / Swiss-Prot | P16885 (Human) | Q8CIH5 (Mouse) | P24135 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1383R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
