| Field | Specification |
|---|---|
| Alternative Names | PLK1;PLK;Serine/threonine-protein kinase PLK1;Polo-like kinase 1;PLK-1;Serine/threonine-protein kinase 13;STPK13 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLK1 around the phosphorylation site of Ser137. AA range:103-152 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PLK1 (Phospho Ser137) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PLK1. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | PLK1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | Phospho-Plk1 (S137) Polyclonal Antibody detects endogenous levels of Plk1 protein only when phosphorylated at S137. |
| Molecular Weight | Observed: 68 kDa |
| UniProt / Swiss-Prot | P53350 (Human) | Q07832 (Mouse) | Q62673 (Rat) |
Recommended dilutions: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human PLK1 around the phosphorylation site of Ser137. AA range:103-152
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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