| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 95 kDa melanocyte specific secreted glycoprotein;95 kDa melanocyte-specific secreted glycoprotein;D12S53E;gp100;M-beta;ME20;ME20 M/ME20 S;ME20-M;ME20-S;ME20M;ME20M/ME20S;ME20S;Melanocyte lineage specific antigen GP100;Melanocyte protein mel 17;Melanocyte protein Pmel 17;Melanocyte protein Pmel 17 precursor;Melanocytes lineage-specific antigen GP100;Melanoma associated ME20 antigen;Melanoma gp100;Melanoma-associated ME20 antigen;Melanosomal matrix protein 17;Melanosomal matrix protein17;P1;p100;p26;PMEL 17;PMEL;PMEL_HUMAN;PMEL17;Premelanosome protein;Secreted melanoma-associated ME20 antigen;SI;SIL;SILV;Silver;Mouse homolog;like;Silver homolog;Silver locus protein homolog;Silver, Mouse, homolog of |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthesized peptide derived from human PMEL AA range: 400-500 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PMEL/Melanoma gp100 (HMB45) (ABT255) Mouse Monoclonal Antibody (WM1457) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against HMB45. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | HMB45 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT255 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human PMEL protein. |
| Molecular Weight | Calculated: 70 kDa | Observed: 80 kDa |
| UniProt / Swiss-Prot | P40967 (Human) | Q60696 (Mouse) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human PMEL AA range: 400-500
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
PMEL is a type I transmembrane glycoprotein which is specific in malanocyte. PMEL is abundantly expressed in melanosomes, and plays a central role in the biogenesis of melanosomes by involved in the maturation of melanosomes from stage I to II. It is highly expressed in all stages of melanoma progression, so the PMEL antibody can specifically labels melanoma cells.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT255.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
