| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Podoplanin AA range: 23-100 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Podoplanin (ABT513) Mouse Monoclonal Antibody (WM1413) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Podoplanin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Podoplanin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT513 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Podoplanin protein. In western blotting of U2O2 cell lysate, the antibody can label a 36 kDa band corresponding to Podoplanin. |
| Molecular Weight | Calculated: 24 kDa | Observed: 36 kDa |
| UniProt / Swiss-Prot | Q86YL7 (Human) | Q62011 (Mouse) | Q64294 (Rat) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Podoplanin AA range: 23-100
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Podoplanin, also known as D2-40 or M2A, is a 38 kDa type I transmembrane mucin with multiple O-glycosylation sites. It is highly expressed in lymphatic endothelial cells, but not in blood vessels. In addition, it is also expressed in a variety of cells, such as mesothelial cells and type I alveolar epithelial cells, follicular dendritic cells, reticular cells, ovarian and testicular germ cells. Podoplanin is a very useful marker to distinguish malignant mesothelioma and adenocarcinoma, seminoma and follicular dendritic cell sarcoma. It is also the main choice to show and quantify lymphatic and intravascular tumor infiltration.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT513.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
