| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PPP4C;PPP4;PPX;Serine/threonine-protein phosphatase 4 catalytic subunit;PP4C;Pp4;Protein phosphatase X;PP-X |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant human PPX protein fragments expressed in E.coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® PPX Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PPX. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Mouse, Rat, Bovine, Dog, Zebrafish.
Validated Applications
| Target | PPX |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Zebrafish |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | PPX Monoclonal Antibody detects endogenous levels of PPX protein. |
| Molecular Weight | Calculated: 35 kDa |
| UniProt / Swiss-Prot | P60510 (Human) | P97470 (Mouse) | Q5BJ92 (Rat) |
Recommended dilutions: WB 1:1000-1:2000;IHC 1:500-1:1000;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human PPX protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Bovine, Dog, Zebrafish. Confirm suitability for untested species before use.
Scientific Background
catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDC2/Cdk1 phosphorylation sites and negatively regulates CDC2/Cdk1 activity in interphase.,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.,subunit:Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;IHC 1:500-1:1000;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Bovine, Dog, Zebrafish. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
