| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Purity | |
| Source | Recombinant |
| Storage |
Product Overview
U-Blot® Prestained Protein Ladder (1.7-40 kDa) contains 6 highly purified prestained recombinant proteins (1.7, 4.6, 10, 15, 25, 40 kDa). Reference bands are color-coded (40 kDa orange-red, 10 kDa green).
The broad molecular-weight range allows dynamic observation of protein migration during electrophoresis and precise estimation of target-protein size. After SDS-PAGE, the prestained bands transfer to PVDF or NC membranes as clearly visible colored bands, allowing transfer efficiency to be monitored directly during Western blotting.
Specifications
| Format | Prestained recombinant proteins (ready-to-use) |
|---|---|
| Number of Bands | 6 |
| Molecular Weight Range | 1.7-40 kDa |
| Band Sizes | 1.7, 4.6, 10, 15, 25, 40 kDa |
| Reference Band Colors | 40 kDa orange-red, 10 kDa green |
| Source | Recombinant |
| Purity | Highly purified |
| Recommended Load | ~5 µl per lane |
| Storage | Stable at -20°C for 36 months; 4°C for 3 months; 25°C for 4 weeks |
Applications
Designed for SDS-PAGE polyacrylamide gel electrophoresis and Western blotting and ELISA, serving as a molecular-weight reference and a visual indicator of transfer performance.
Compatibility & Usage Notes
Ready to use: do not boil, dilute, or add reducing agents. Prestained proteins show different apparent molecular weights in different buffer systems; use as a reference relative to unstained standards when precise sizing is required.
Safety & Handling
Instructions for Use
- This product is ready to use. Thaw at room temperature for a few minutes, then gently mix. Do not boil, dilute, or add reducing agents.
- Load 5 µl into the gel wells (increase for wide wells). For first-time use, optimize the load with a pilot run.
- For frequent use, store at 2-8°C (3-month shelf life); aliquot to avoid repeated thawing.
Technical Notes
- Watch electrophoresis time; low-MW proteins may migrate off the gel front.
- For Western blotting of small molecules, bands below ~3 kDa may pass through the membrane.
- Avoid SDS in the transfer buffer; if required, keep below 0.02-0.04%.
- Prestained proteins show different apparent molecular weights in different buffer systems; pre-calibrate with non-prestained proteins for approximate sizing.
Migration Pattern
Migration pattern of U-Blot® Prestained Protein Ladder (1.7-40 kDa): apparent molecular weights (kDa) are determined by calibration against unstained protein standards in various electrophoresis buffer systems.
Yes. Thaw at room temperature for a few minutes and mix gently before loading. Do not boil, dilute, or add reducing agents.
It spans 1.7-40 kDa across 6 bands (1.7, 4.6, 10, 15, 25, 40 kDa).
40 kDa orange-red, 10 kDa green, providing visual reference points on the gel and membrane.
Approximately 5 µl per lane; increase slightly for wide wells. Run a pilot to optimize for your system.
Stable at -20°C for 36 months; 4°C for 3 months; 25°C for 4 weeks Aliquot and avoid repeated freeze-thaw for best performance.
BioHippo can source custom configurations, bulk volumes, and special packaging for this product through our manufacturing partner. Contact us with your requirements for a quote and lead time.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
