| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PRMT6;HRMT1L6;Protein arginine N-methyltransferase 6;Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6;Histone-arginine N-methyltransferase PRMT6 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human PRMT6 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® PRMT6 Mouse Monoclonal Antibody (WM0527) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PRMT6. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Mouse, Rat.
Validated Applications
| Target | PRMT6 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | PRMT6 Monoclonal Antibody detects endogenous levels of PRMT6 protein. |
| Molecular Weight | Calculated: 42 kDa |
| UniProt / Swiss-Prot | Q96LA8 (Human) | Q6NZB1 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;IHC 1:500-1:1000;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human PRMT6 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;IHC 1:500-1:1000;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
