| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ATLD2;cb16;Cyclin;DNA polymerase delta auxiliary protein;etID36690.10;fa28e03;fb36g03;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating cell nuclear antigen;wu:fa28e03;wu:fb36g03 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Proliferating Cell Nuclear Antigen AA range: 1-100 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Proliferating Cell Nuclear Antigen (ABT221) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PCNA. It has been validated for Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | PCNA |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT221 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody can specifically recognize human PCNA protein. |
| Molecular Weight | Calculated: 29 kDa | Observed: 35 kDa |
| UniProt / Swiss-Prot | P12004 (Human) | P17918 (Mouse) | P04961 (Rat) |
Recommended dilutions: IHC 1:200-400;ELISA 1:500-5000
Immunogen / Antigen
Synthesized peptide derived from human Proliferating Cell Nuclear Antigen AA range: 1-100
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, ELISA. Recommended dilutions: IHC 1:200-400;ELISA 1:500-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT221.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
