| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type |
Product Overview
U-Blot® PVDF Transfer Membrane is a polyvinylidene fluoride (PVDF) microporous membrane designed for protein transfer from various gel matrices. Compared to nitrocellulose membranes, it offers improved processing characteristics and mechanical strength. It is available in two pore-size variants (0.45 µm and 0.2 µm), enabling binding of proteins across a broad molecular-weight range.
The 0.45 µm pore size is suitable for most proteins and offers high detection sensitivity, while the 0.2 µm pore size is ideal for proteins with a molecular weight below 20,000, enabling superior adsorption and retention of small-molecule proteins. The membrane exhibits excellent protein retention, high physical strength, and broad chemical compatibility, making it well-suited for immunological assays.
Specifications
| Material | PVDF |
|---|---|
| Membrane Property | Hydrophobic |
| Pore Sizes | 0.2 µm and 0.45 µm |
| Protein Binding Capacity (BSA) | >260 µg/cm² (0.2 µm); >180 µg/cm² (0.45 µm) |
| Format / Dimensions | 26.5 cm x 3.75 m |
| Detection Methods | Chemiluminescent, Colorimetric, Radioactive |
| Applications | Western Blot, Northern Blot, Southern Blot |
Applications
Suitable for Western blot, Northern blot, and Southern blot workflows, with compatibility across chemiluminescent, colorimetric, and radioactive detection systems.
Compatibility & Usage Notes
Select the 0.45 µm pore size for most proteins and high sensitivity; choose the 0.2 µm pore size for proteins below ~20 kDa where superior retention of small proteins is needed.
Safety & Handling
Transfer Protocol
- Separate protein samples and markers by SDS-PAGE.
- Cut and equilibrate the gel in cold transfer buffer for 5-20 minutes; cut membrane and filter paper to size.
- Pre-wet the PVDF membrane in methanol for ~20 seconds until uniformly translucent gray, soak in ultrapure water for 1-2 minutes, then equilibrate in cold transfer buffer for 2-3 minutes. Do not let the membrane dry; re-wet if it turns opaque white.
- Assemble the transfer sandwich: clamp -> sponge pad -> filter paper -> pre-wetted membrane -> pre-wetted gel -> filter paper -> sponge pad -> clamp, avoiding wrinkles and bubbles.
- Set the operating conditions per your transfer device and start the transfer.
- After transfer, remove the blot, rinse briefly with ultrapure water, then air-dry for storage or proceed directly to immunodetection.
Handling Instructions
- Wear gloves to prevent fingerprint contamination.
- Use blunt forceps to prevent membrane damage.
- Keep the light-blue protective paper with the membrane while cutting; discard it after wetting.
- Handle carefully to avoid scratching the surface and do not fold the membrane.
- After transfer, rinse the blot with ultrapure water to remove residual impurities.
- The blot can be air-dried and stored at 4°C for several months, or used immediately.
Recommended Membrane by Application
- General immunological testing → PVDF or NC Transfer Membrane (0.45 µm)
- Amino-acid analysis → PVDF Transfer Membrane (0.45 µm)
- Detection of low-abundance or small-molecular-weight proteins → PVDF or NC Transfer Membrane (0.2 µm)
- Sequencing of low-abundance or low-MW proteins → PVDF Transfer Membrane (0.2 µm)
The 0.45 µm pore size suits most proteins and offers high detection sensitivity, while the 0.2 µm pore size is ideal for proteins below about 20 kDa, giving superior retention of small proteins.
Yes. Being hydrophobic, it must be pre-wet in methanol for ~20 seconds until uniformly translucent, then rinsed in ultrapure water and equilibrated in cold transfer buffer. Do not let it dry out after wetting.
The membrane is compatible with chemiluminescent, colorimetric, and radioactive detection systems.
Always wear gloves and use blunt forceps, avoid folding or scratching the surface, and keep the membrane wet throughout immunodetection.
The membrane is supplied in roll format with dimensions of 26.5 cm x 3.75 m, in 0.2 µm and 0.45 µm pore-size variants.
BioHippo can source custom configurations, bulk volumes, and special packaging for this product through our manufacturing partner. Contact us with your requirements for a quote and lead time.
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