| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human pVHL AA range: 150-213 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® pVHL (ABT-PVHL) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against VHL. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | VHL |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-PVHL |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of pVHL protein. |
| Molecular Weight | Calculated: 24 kDa,19 kDa | Observed: 17 kDa |
| UniProt / Swiss-Prot | P40337 (Human) |
Recommended dilutions: IHC 1:50-200;IF 1:50-200;ELISA 1:500-5000
Immunogen / Antigen
Synthesized peptide derived from human pVHL AA range: 150-213
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
von Hippel-Lindau tumor suppressor(VHL) Homo sapiens Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:50-200;IF 1:50-200;ELISA 1:500-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-PVHL.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
