| Field | Specification |
|---|---|
| Target | |
| Alternative names | mitochondrial;ODPA_HUMAN;PDH;PDHA;PDHA1;PDHCE1A;PDHE1 A type I;PDHE1-A type I;PHE1A;Pyruvate Dehydrogenase;lipoamide;alpha 1;Pyruvate dehydrogenase complex, E1 alpha polypeptide 1;Pyruvate Dehydrogenase E1 alpha;Pyruvate dehydrogenase E1 component subunit alpha;Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;somatic form. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® pyruvate dehydrogenase (lipoamide) α 1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against PDHA1. It has been validated for Western Blot, Immunofluorescence and reacts with Human, Mouse.
Validated Applications
| Target | PDHA1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of pyruvate dehydrogenase (lipoamide) alpha 1 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 43 kDa |
| UniProt / Swiss-Prot | P08559 (Human) | P35486 (Mouse) |
Recommended dilutions: WB 1:1000;ICC 1:100
Immunogen / Antigen
Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000;ICC 1:100
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
