U-Blot® RARα Rabbit Polyclonal Antibody

SKU:BHA21808978
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-RARα antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP14218-50 50 uL
WP14218-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WP14218
Alternative Names RARA;NR1B1;Retinoic acid receptor alpha;RAR-alpha;Nuclear receptor subfamily 1 group B member 1
Antigen
  • Retinoic acid receptor alpha
Cellular Localization Nucleus . Cytoplasm . Nuclear localization depends on ligand binding, phosphorylation and sumoylation (PubMed:19850744). Translocation to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation (By similarity). Increased nuclear localization upon pulsatile shear stress (PubMed:28167758). .
Clonality
  • Polyclonal
Concentration 1 mg/ml
Conjugate
  • Unconjugated
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5914 (Human) | 19401 (Mouse)
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha. AA range:46-95
Isotype
  • IgG
Molecular Weight Observed: 51 kDa
Product Type
  • Polyclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
  • Mouse
  • Rat
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target RARα
Antibody

Product Overview

U-Blot® RARα Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RARα. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target RARα
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity RARα Polyclonal Antibody detects endogenous levels of RARα protein.
Molecular Weight Observed: 51 kDa
UniProt / Swiss-Prot P10276 (Human) | P11416 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha. AA range:46-95

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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