| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | APEX1;APE;APE1;APEX;APX;HAP1;REF1;DNA-;apurinic or apyrimidinic site;lyase;APEX nuclease;APEN;Apurinic-apyrimidinic endonuclease 1;AP endonuclease 1;APE-1;REF-1;Redox factor-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys7. AA range:1-50 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Ref-1 (Acetyl Lys7) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Ref-1. Recognizes Acetyl. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Ref-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | Acetyl-Ref-1 (K7) Polyclonal Antibody detects endogenous levels of Ref-1 protein only when acetylated at K7. |
| Molecular Weight | Observed: 35 kDa |
| UniProt / Swiss-Prot | P27695 (Human) | P28352 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys7. AA range:1-50
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
