U-Blot® Repo-Man Rabbit Polyclonal Antibody

SKU:BHA21809019
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Repo-Man antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human.
Clonality Polyclonal
Host Rabbit
Reactivity Human
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP14255-50 50 uL
WP14255-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WP14255
Alternative Names CDCA2;Cell division cycle-associated protein 2;Recruits PP1 onto mitotic chromatin at anaphase protein;Repo-Man
Antigen
  • Cell division cycle-associated protein 2
Cellular Localization Nucleus . Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.
Clonality
  • Polyclonal
Concentration 1 mg/ml
Conjugate
  • Unconjugated
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 157313 (Human)
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human CDCA2. AA range:511-560
Isotype
  • IgG
Molecular Weight Observed: 120-130 kDa
Product Type
  • Polyclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target Repo-Man
Antibody

Product Overview

U-Blot® Repo-Man Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Repo-Man. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.

Validated Applications

Target Repo-Man
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Repo-Man Polyclonal Antibody detects endogenous levels of Repo-Man protein.
Molecular Weight Observed: 120-130 kDa
UniProt / Swiss-Prot Q69YH5 (Human) | Q14B71 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human CDCA2. AA range:511-560

Reactivity Notes

Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.

Scientific Background

cell division cycle associated 2(CDCA2) Homo sapiens This gene encodes a targeting subunit of the cell-cycle associated protein, protein phosphatase 1, with a role in targeting this protein to chromatin during anaphase. These two proteins comprise a phosphatase complex that is involved in nuclear envelope reformation and regulation of the DNA damage response. The encoded protein may also play a role in cancer progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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