| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Replication protein A 70 kDa DNA-binding subunit;RP-A p70;Replication factor A protein 1;RF-A protein 1;Single-stranded DNA-binding protein |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human RFA1 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
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| Catalog no. (Mfr.) | |
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Product Overview
U-Blot® RFA1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RFA1. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Monkey, Mouse, Rat.
Validated Applications
| Target | RFA1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Monkey, Mouse, Rat |
| Applications | Western Blot, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human,Monkey,Mouse,Rat RFA1 |
| Molecular Weight | Calculated: 68 kDa |
| UniProt / Swiss-Prot | P27694 (Human) | Q8VEE4 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Immunogen / Antigen
Synthesized peptide derived from human RFA1
Reactivity Notes
Confirmed cross-reactivity: Human, Monkey, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
function:It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the replication factor A protein 1 family.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Confirmed reactivity: Human, Monkey, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
