| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | RFC2;Replication factor C subunit 2;Activator 1 40 kDa subunit;A1 40 kDa subunit;Activator 1 subunit 2;Replication factor C 40 kDa subunit;RF-C 40 kDa subunit;RFC40 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFC2. AA range:131-180 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® RFC2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RFC2. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | RFC2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | RFC2 Polyclonal Antibody detects endogenous levels of RFC2 protein. |
| Molecular Weight | Observed: 40 kDa |
| UniProt / Swiss-Prot | P35250 (Human) | Q9WUK4 (Mouse) | Q641W4 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human RFC2. AA range:131-180
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
