U-Blot® RFWD3 Rabbit Polyclonal Antibody

SKU:BHA21809030
New
Suppliers
Catalog / Quick links
    Overview
    Click light‑blue chips for details
    Anti-RFWD3 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse.
    Clonality Polyclonal
    Host Rabbit
    Reactivity Human, Mouse
    Conjugate Unconjugated
    Isotype IgG
    Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
    Options selector
    Catalog no. Size
    WP14265-50 50 uL
    WP14265-100 100 uL
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Size: 50 uL
    • Lead time: options listed in "Availability Content"; other statuses may take longer.
    • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: store at recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Field Specification
    Alternative Names RFWD3;RNF201;E3 ubiquitin-protein ligase RFWD3;RING finger and WD repeat domain-containing protein 3;RING finger protein 201
    Antigen
    • E3 ubiquitin-protein ligase RFWD3
    Cellular Localization Nucleus . Nucleus, PML body . Cytoplasm . In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276). In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657). This recruitment depends upon RPA2 (PubMed:21504906). .
    Clonality
    • Polyclonal
    Concentration 1 mg/ml
    Conjugate
    • Unconjugated
    Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Gene ID 55159 (Human)
    Host Rabbit
    Immunogen The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423
    Isotype
    • IgG
    Molecular Weight Observed: 85 kDa
    Product Type
    • Polyclonal Antibody
    • Primary Antibodies
    Reactivity
    • Human
    • Mouse
    Storage -15°C to -25°C/1 year(Do not lower than -25°C)
    Target RFWD3
    Antibody

    Product Overview

    U-Blot® RFWD3 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RFWD3. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.

    Validated Applications

    Target RFWD3
    Host Rabbit
    Clonality Polyclonal
    Isotype IgG
    Conjugate Unconjugated
    Reactivity Human, Mouse
    Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
    Purification Affinity Purified
    Specificity RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.
    Molecular Weight Observed: 85 kDa
    UniProt / Swiss-Prot Q6PCD5 (Human) | Q8CIK8 (Mouse)

    Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

    Immunogen / Antigen

    The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423

    Reactivity Notes

    Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

    Scientific Background

    PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 3 WD repeats.

    Safety & Handling

    For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
    What applications is this antibody validated for?

    This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

    What species does it react with?

    Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

    Is this antibody conjugated?

    Conjugate: Unconjugated.

    How should it be stored?

    Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

    Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

    Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.

    🔬 What's on offer right now:

    10% Off Pre-Designed siRNA Sets

    15% Off Cancer Antibodies

    20% Off Transmembrane Proteins

    $50 Off All ELISA Kits

    50% Off Lab Consumables + Free Shipping

    $99 Pipette Filler Promotion Package

    BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer

    DENARASE® Endonuclease — 10% Off One Order

    10% OFF CELL LINES-Limited-Time Offer

    Free Sample – CellTrypase Recombinant Trypsin-Like Enzyme

    Get a Quote

    Please use this form for bulk quantity requests or customized products.

    Contact Information

    Product Information

    Try Celltrypse Free – Request Your Sample Today

    Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

    Try Celltrypse Free – Request Your Sample Today

    Try Celltrypse Free – Request Your Sample Today

    Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

    Try Celltrypse Free – Request Your Sample Today