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| Antigen | |
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| Isotype | |
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| Applications | |
| Immunogen | Synthesized peptide derived from human RFX5 AA range: 491-541 |
| Conjugate | |
| Molecular weight | |
| Purification | |
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Product Overview
U-Blot® RFX5 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RFX5. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Mouse.
Validated Applications
| Target | RFX5 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of RFX5 at Human/Mouse |
| Molecular Weight | Calculated: 68 kDa |
| UniProt / Swiss-Prot | P48382 (Human) | Q9JL61 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
Synthesized peptide derived from human RFX5 AA range: 491-541
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
