| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Transforming protein RhoA;Rho cDNA clone 12;h12 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Rho A |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Rho A Mouse Monoclonal Antibody (4E12) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Rho A. It has been validated for Immunohistochemistry, Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Rho A |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4E12 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Rho A at Human, Mouse,Rat |
| Molecular Weight | Observed: 22 kDa |
| UniProt / Swiss-Prot | P61586 (Human) | Q9QUI0 (Mouse) | P61589 (Rat) |
Recommended dilutions: IHC1:50-200;WB 1:1000-2000
Immunogen / Antigen
Synthesized peptide derived from human Rho A
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: IHC1:50-200;WB 1:1000-2000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 4E12.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
