| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RHOA;ARH12;ARHA;RHO12;Transforming protein RhoA;Rho cDNA clone 12;h12 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RhoA. AA range:144-193 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Rho A Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Rho A. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Rho A |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Rho A Polyclonal Antibody detects endogenous levels of Rho A protein. |
| Molecular Weight | Observed: 22 kDa |
| UniProt / Swiss-Prot | P61586 (Human) | Q9QUI0 (Mouse) | P61589 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human RhoA. AA range:144-193
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
