U-Blot® RhoA (Phospho Ser188) Rabbit Polyclonal Antibody

SKU:BHA21804953
New
—
Suppliers
UcallM Biosciences Inc
UcallM Biosciences Inc
Details Products
Overview
Click light‑blue chips for details
Anti-RhoA antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA, Immunohistochemistry with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA, Immunohistochemistry
Options selector
Catalog no. Size
WP10558-50 50 uL
WP10558-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target RhoA
Antigen
  • RhoA (Phospho Ser188)
Alternative names Transforming protein RhoA;Rho cDNA clone 12;h12
Gene ID 387 (Human) | 11848 (Mouse) | 117273 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
  • Immunohistochemistry
Immunogen Synthesized peptide derived from human RhoA (Phospho Ser188)
Conjugate
  • Unconjugated
Molecular weight Observed: 22 kDa
Purification Affinity Purified
Cellular localization Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP10558
Main SKU BHA21804953
Antibody

Product Overview

U-Blot® RhoA (Phospho Ser188) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RhoA. Recognizes Phospho. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.

Validated Applications

Target RhoA
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA, Immunohistochemistry
Purification Affinity Purified
Specificity This antibody detects endogenous levels of Human,Mouse,Rat RhoA (Phospho Ser188)
Molecular Weight Observed: 22 kDa
UniProt / Swiss-Prot P61586 (Human) | Q9QUI0 (Mouse) | P61589 (Rat)

Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

Immunogen / Antigen

Synthesized peptide derived from human RhoA (Phospho Ser188)

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today