U-Blot® RhoA Rabbit Monoclonal Antibody

SKU:BHA21800031
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Rho A antibody (Rabbit host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Monoclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG, Kappa
Clone PT0088R
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WR4031-50 50 uL
WR4031-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WR4031
Alternative Names Transforming protein RhoA;Rho cDNA clone 12;h12
Antigen
  • Rho A
Cellular Localization Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .
Clonality
  • Monoclonal
Conjugate
  • Unconjugated
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Gene ID 387 (Human) | 11848 (Mouse) | 117273 (Rat)
Host Rabbit
Isotype
  • IgG
  • Kappa
Molecular Weight Calculated: 21 kDa | Observed: 21 kDa
Product Type
  • Monoclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
  • Mouse
  • Rat
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target Rho A
Antibody

Product Overview

U-Blot® RhoA Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Rho A. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target Rho A
Host Rabbit
Clonality Monoclonal
Clone PT0088R
Isotype IgG, Kappa
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Protein A
Specificity Endogenous
Molecular Weight Calculated: 21 kDa | Observed: 21 kDa
UniProt / Swiss-Prot P61586 (Human) | Q9QUI0 (Mouse) | P61589 (Rat)

Recommended dilutions: IHC 1:200-1000;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000

Immunogen / Antigen

Rho A

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone PT0088R.

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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