| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RPL5;MSTP030;60S ribosomal protein L5 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPL5. AA range:161-210 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Ribosomal Protein L5 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Ribosomal Protein L5. It has been validated for Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Ribosomal Protein L5 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Ribosomal Protein L5 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L5 protein. |
| Molecular Weight | Observed: 34 kDa |
| UniProt / Swiss-Prot | P46777 (Human) | P47962 (Mouse) | P09895 (Rat) |
Recommended dilutions: WB 1:500-2000;IP 1:50-200;IHC 1:100-500;IF/ICC1:100-500;ELISA 1:5000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human RPL5. AA range:161-210
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found
Safety & Handling
This antibody is validated for Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IP 1:50-200;IHC 1:100-500;IF/ICC1:100-500;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
