| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Receptor-interacting serine/threonine-protein kinase 1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP |
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| Host | |
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Product Overview
U-Blot® RIPK1 (Phospho Ser166) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against RIPK1 Phospho Ser166. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | RIPK1 Phospho Ser166 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0757R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | RIPK1 (Phospho Ser166) Antibody detects endogenous levels of RIPK1 protein only when phosphorylated at S166.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):MWsKL |
| Molecular Weight | Calculated: 76 kDa | Observed: 80 kDa |
| UniProt / Swiss-Prot | Q13546 (Human) | Q60855 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Receptor-interacting serine/threonine-protein kinase 1 (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP)
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0757R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
