| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DROSHA;RN3;RNASE3L;RNASEN;Ribonuclease 3;Protein Drosha;Ribonuclease III;RNase III;p241 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNase III Drosha. AA range:774-823 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® RNase III Drosha Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RNase III Drosha. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | RNase III Drosha |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | RNase III Drosha Polyclonal Antibody detects endogenous levels of RNase III Drosha protein. |
| Molecular Weight | Observed: 160 kDa |
| UniProt / Swiss-Prot | Q9NRR4 (Human) | Q5HZJ0 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human RNase III Drosha. AA range:774-823
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
drosha ribonuclease III(DROSHA) Homo sapiens This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
