| Field | Specification |
|---|---|
| Target | |
| Alternative names | 60S acidic ribosomal protein P1;AA409079;AI325195;AU020965;HSSB;ik:tdsubc_2g1;M;2;21C;MGC137236;OTTHUMP00000004008;p32;p34;RCJMB04_6d17 replication protein A2, 32kDa;REPA 2;REPA1;REPA2;Replication factor A protein 2;Replication protein A 32 kDa subunit;Replication protein A 32kDa subunit;Replication protein A 34 kDa subunit;Replication protein A;replication protein A1;70kD;Replication Protein A2;32kDa;Replication protein A2 32kD;Replication protein A2 32kDa;Replication protein A2;Replication protein A2, 32kDa;RF A;RF-A protein 2;Rf-A2;RFA;RFA2_HUMAN;RP A;RP-A p32;RP-A p34;RP21C;RPA 2;RPA 32;RPA;RPA2;RPA32;RPA34;RPA70;RpLP1;RpP2;xx:tdsubc_2g1;zgc:109822. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® RPA32/RPA2 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against RFA2. It has been validated for Western Blot, Immunoprecipitation, Immunofluorescence and reacts with Human.
Validated Applications
| Target | RFA2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunoprecipitation, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of RPA32/RPA2 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 32 kDa |
| UniProt / Swiss-Prot | P15927 (Human) | Q62193 (Mouse) |
Recommended dilutions: WB 1:2000;ICC 1:200
Immunogen / Antigen
Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
Safety & Handling
This antibody is validated for Western Blot, Immunoprecipitation, Immunofluorescence. Recommended dilutions: WB 1:2000;ICC 1:200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
